› 2eua

X-ray diffraction
2.5Å resolution

Structure and Mechanism of MenF, the Menaquinone-Specific Isochorismate Synthase from Escherichia Coli

Released:
DOI: 10.2210/pdb2eua/pdb
PDB entry 2eua coloured by chain, front view.
PDB entry 2eua coloured by chain, side view.
PDB entry 2eua coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Lysine 190 is the catalytic base in MenF, the menaquinone-specific isochorismate synthase from Escherichia coli: implications for an enzyme family.
Kolappan S, Zwahlen J, Zhou R, Truglio JJ, Tonge PJ, Kisker C
Biochemistry 46 946-53 (2007)
PMID: 17240978

Function and Biology Details

Reaction catalysed: 
Chorismate = isochorismate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isochorismate synthase MenF Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 48.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P38051 (Residues: 1-431; Coverage: 100%)
Gene names: JW2260, b2265, menF, yfbA
Sequence domains: chorismate binding enzyme
Structure domains: Anthranilate synthase

Ligands and Environments

1 bound ligand:
Ligand TAR 2 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P43212
Unit cell:
a: 146.408Å b: 146.408Å c: 125.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.276
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study.
Ronnebaum et al. (2018)
4 more

2 mentions without citation

Comparative analysis of plant isochorismate synthases reveals structural mechanisms underlying their distinct biochemical properties.
Yokoo et al. (2018)
1 more