2erh

X-ray diffraction
2Å resolution

Crystal Structure of the E7_G/Im7_G complex; a designed interface between the colicin E7 DNAse and the Im7 immunity protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-169934 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E7 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03708 (Residues: 1-87; Coverage: 100%)
Gene names: ceiE7, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E7 Chain: B
Molecule details ›
Chain: B
Length: 127 amino acids
Theoretical weight: 14.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 447-573; Coverage: 22%)
Gene names: cea, colE7
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: I222
Unit cell:
a: 61.038Å b: 72.969Å c: 121.167Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.28 0.228 0.271
Expression system: Escherichia coli