› 2e9x

X-ray diffraction
2.3Å resolution

The crystal structure of human GINS core complex

Released:
DOI: 10.2210/pdb2e9x/pdb
PDB entry 2e9x coloured by chain, front view.
PDB entry 2e9x coloured by chain, side view.
PDB entry 2e9x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human GINS complex and its assembly and functional interface in replication initiation.
Kamada K, Kubota Y, Arata T, Shindo Y, Hanaoka F
Nat Struct Mol Biol 14 388-96 (2007)
PMID: 17417653

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-172086 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
DNA replication complex GINS protein PSF1 Chains: A, E
Molecule details ›
Chains: A, E
Length: 149 amino acids
Theoretical weight: 17.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14691 (Residues: 1-149; Coverage: 76%)
Gene names: GINS1, KIAA0186, PSF1
Sequence domains: GINS complex protein
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
DNA replication complex GINS protein PSF2 Chains: B, F
Molecule details ›
Chains: B, F
Length: 185 amino acids
Theoretical weight: 21.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y248 (Residues: 1-185; Coverage: 100%)
Gene names: CGI-122, DC5, GINS2, HSPC037, PSF2
Sequence domains: GINS complex protein
Structure domains:
DNA replication complex GINS protein PSF3 Chains: C, G
Molecule details ›
Chains: C, G
Length: 219 amino acids
Theoretical weight: 24.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BRX5 (Residues: 1-216; Coverage: 100%)
Gene names: GINS3, PSF3
Sequence domains: GINS complex protein
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
DNA replication complex GINS protein SLD5 Chains: D, H
Molecule details ›
Chains: D, H
Length: 223 amino acids
Theoretical weight: 26.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BRT9 (Residues: 1-223; Coverage: 100%)
Gene names: GINS4, SLD5
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P1
Unit cell:
a: 57.928Å b: 88.741Å c: 102.129Å
α: 104.689° β: 102.964° γ: 95.467°
R-values:
R R work R free
0.199 0.197 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Eukaryotic chromosome DNA replication: where, when, and how?
Masai et al. (2010)
11 more

8 mentions without citation

The architecture of a eukaryotic replisome.
Sun et al. (2015)
7 more