› 2e7p

X-ray diffraction
2.1Å resolution

Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides

Released:
DOI: 10.2210/pdb2e7p/pdb
PDB entry 2e7p coloured by chain, front view.
PDB entry 2e7p coloured by chain, side view.
PDB entry 2e7p coloured by chain, top view.
Source organism: Populus tremula x Populus tremuloides
Primary publication:
Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1.
Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP
Proc Natl Acad Sci U S A 104 7379-84 (2007)
PMID: 17460036

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaredoxin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 116 amino acids
Theoretical weight: 12.44 KDa
Source organism: Populus tremula x Populus tremuloides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5PSJ1 (Residues: 11-126; Coverage: 92%)
Gene name: grxC1
Sequence domains: Glutaredoxin
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand FES 1 x FES
Ligand GSH 4 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU, PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P61
Unit cell:
a: 97.774Å b: 97.774Å c: 91.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.22
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Ascorbate and glutathione: the heart of the redox hub.
Foyer et al. (2011)
26 more

11 mentions without citation

Glutaredoxins: roles in iron homeostasis.
Rouhier et al. (2010)
10 more