› 2e6f

X-ray diffraction
1.26Å resolution

Crystal structure of Trypanosoma cruzi dihydroorotate dehydrogenase in complex with oxonate

Released:
DOI: 10.2210/pdb2e6f/pdb
PDB entry 2e6f coloured by chain, front view.
PDB entry 2e6f coloured by chain, side view.
PDB entry 2e6f coloured by chain, top view.
Source organism: Trypanosoma cruzi
Entry authors: Inaoka DK, Shimizu H, Sakamoto K, Shiba T, Kurisu G, Nara T, Aoki T, Harada S, Kita K

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + fumarate = orotate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (fumarate) Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 34.2 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q4D3W2 (Residues: 1-314; Coverage: 100%)
Gene names: Tc00.1047053508375.50, pyr4, tcdhod2
Sequence domains: Dihydroorotate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
3 bound ligands:
Ligand NCO 1 x NCO
Ligand OXC 2 x OXC
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 68.359Å b: 71.828Å c: 123.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 0.18
Expression system: Escherichia coli BL21

Quick links