› 2e3r

X-ray diffraction
1.65Å resolution

Crystal structure of CERT START domain in complex with C18-ceramide (P1)

Released:
DOI: 10.2210/pdb2e3r/pdb
PDB entry 2e3r coloured by chain, front view.
PDB entry 2e3r coloured by chain, side view.
PDB entry 2e3r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide.
Kudo N, Kumagai K, Tomishige N, Yamaji T, Wakatsuki S, Nishijima M, Hanada K, Kato R
Proc Natl Acad Sci U S A 105 488-93 (2008)
PMID: 18184806

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ceramide transfer protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5P4 (Residues: 347-624; Coverage: 40%)
Gene names: CERT, CERT1, COL4A3BP, STARD11
Sequence domains: START domain
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand 18C 2 x 18C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 46.459Å b: 50.134Å c: 62.652Å
α: 77.54° β: 70.96° γ: 81.85°
R-values:
R R work R free
0.194 0.192 0.226
Expression system: Escherichia coli

Quick links

Citations

50 review citations

An overview of sphingolipid metabolism: from synthesis to breakdown.
Gault et al. (2010)
49 more

5 mentions without citation

Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide.
Kudo et al. (2008)
4 more