› 2dzu

X-ray diffraction
2.46Å resolution

Structure of mutant tryptophan synthase alpha-subunit (D110N) from a hyperthermophile, Pyrococcus furiosus

Released:
DOI: 10.2210/pdb2dzu/pdb
PDB entry 2dzu coloured by chain, front view.
PDB entry 2dzu coloured by chain, side view.
PDB entry 2dzu coloured by chain, top view.
Source organism: Pyrococcus furiosus
Entry authors: Ogasahara K, Yamagata Y, Yutani K, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase alpha chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 27.53 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U094 (Residues: 1-248; Coverage: 100%)
Gene names: PF1705, trpA
Sequence domains: Tryptophan synthase alpha chain
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: C2221
Unit cell:
a: 73.254Å b: 77.474Å c: 166.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.26
Expression system: Escherichia coli

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