› 2du1

X-ray diffraction
2.6Å resolution

Crystal Structure of basic winged bean lectin in complex with Methyl-alpha-N-acetyl-D galactosamine

Released:
DOI: 10.2210/pdb2du1/pdb
PDB entry 2du1 coloured by chain, front view.
PDB entry 2du1 coloured by chain, side view.
PDB entry 2du1 coloured by chain, top view.
Source organism: Psophocarpus tetragonolobus
Primary publication:
Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc.
Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K
Acta Crystallogr D Biol Crystallogr 62 1319-24 (2006)
PMID: 17057334

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Basic agglutinin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 241 amino acids
Theoretical weight: 26.51 KDa
Source organism: Psophocarpus tetragonolobus
UniProt:
  • Canonical: O24313 (Residues: 2-242; Coverage: 100%)
Gene name: WBAI
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand MGC 4 x MGC
Ligand NAG 3 x NAG
Ligand CA 4 x CA
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21212
Unit cell:
a: 157.619Å b: 90.96Å c: 73.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.251

Quick links

Citations

2 citation in other articles

Generation of blood group specificity: new insights from structural studies on the complexes of A- and B-reactive saccharides with basic winged bean agglutinin.
Kulkarni et al. (2007)
1 more