› 2dnj

X-ray diffraction
2Å resolution

DNASE I-INDUCED DNA CONFORMATION. 2 ANGSTROMS STRUCTURE OF A DNASE I-OCTAMER COMPLEX

Released:
DOI: 10.2210/pdb2dnj/pdb
PDB entry 2dnj coloured by chain, front view.
PDB entry 2dnj coloured by chain, side view.
PDB entry 2dnj coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
DNase I-induced DNA conformation. 2 A structure of a DNase I-octamer complex.
Lahm A, Suck D
J. Mol. Biol. 222 645-67 (1991)
PMID: 1748997

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115174 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Deoxyribonuclease-1 Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase
5'-D(*GP*CP*GP*AP*TP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Bos taurus
Expression system: Not provided
5'-D(*GP*CP*GP*AP*TP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 6 nucleotides
Theoretical weight: 1.81 KDa
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 72.9Å b: 100.1Å c: 92.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 not available not available
Expression system: Not provided

Quick links

Citations

8 review citations

Review: nuclear events in apoptosis.
Robertson et al. (2000)
7 more

23 mentions without citation

Evolving insights on how cytosine methylation affects protein-DNA binding.
Dantas Machado et al. (2015)
22 more