› 2dhh

X-ray diffraction
2.8Å resolution

Crystal structure of a multidrug transporter reveal a functionally rotating mechanism

Released:
DOI: 10.2210/pdb2dhh/pdb
PDB entry 2dhh coloured by chain, front view.
PDB entry 2dhh coloured by chain, side view.
PDB entry 2dhh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of a multidrug transporter reveal a functionally rotating mechanism.
Murakami S, Nakashima R, Yamashita E, Matsumoto T, Yamaguchi A
Nature 443 173-9 (2006)
PMID: 16915237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1053 amino acids
Theoretical weight: 114.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 225.87Å b: 134.418Å c: 163.192Å
α: 90° β: 97.71° γ: 90°
R-values:
R R work R free
0.27 0.268 0.307
Expression system: Escherichia coli K-12

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Citations

91 review citations

Molecular mechanisms of antibiotic resistance.
Blair et al. (2015)
90 more

21 mentions without citation

Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes.
Lundbaek et al. (2010)
20 more