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GCN4 BASIC DOMAIN, LEUCINE ZIPPER COMPLEXED WITH ATF/CREB SITE DNA

X-ray diffraction
2.2Å resolution
PDB entry 2dgc coloured by chain, front view.
PDB entry 2dgc coloured by chain, side view.
PDB entry 2dgc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: General control transcription factor GCN4 and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 12889.84 Å2
Buried surface area: 5910.29 Å2
Dissociation area: 1,312.39 Å2
Dissociation energy (ΔGdiss): 28.03 kcal/mol
Dissociation entropy (TΔSdiss): 11.12 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-115171

Macromolecules

General control transcription factor GCN4
Chain: A
Length: 63 amino acids
Theoretical weight: 7.38 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P03069 (Residues: 220-281; Coverage: 22%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Pfam: Basic region leucine zipper
InterPro:
CATH: Classic Zinc Finger
SCOP: Leucine zipper domain
General control transcription factor GCN4 in PDB entry 2dgc, assembly 1, front view.
General control transcription factor GCN4 in PDB entry 2dgc, assembly 1, side view.
General control transcription factor GCN4 in PDB entry 2dgc, assembly 1, top view.
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DNA chain B
Name: DNA (5'-D(*TP*GP*GP*AP*GP*AP*TP*GP*AP*CP*GP*TP*CP*AP*TP*CP*T P*CP*C)-3')
Representative chains: B
Source organism: Saccharomyces cerevisiae [4932]
Expression system: Not provided
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
DNA (5'-D(*TP*GP*GP*AP*GP*AP*TP*GP*AP*CP*GP*TP*CP*AP*TP*CP*T P*CP*C)-3') in PDB entry 2dgc, assembly 1, front view.
DNA (5'-D(*TP*GP*GP*AP*GP*AP*TP*GP*AP*CP*GP*TP*CP*AP*TP*CP*T P*CP*C)-3') in PDB entry 2dgc, assembly 1, side view.
DNA (5'-D(*TP*GP*GP*AP*GP*AP*TP*GP*AP*CP*GP*TP*CP*AP*TP*CP*T P*CP*C)-3') in PDB entry 2dgc, assembly 1, top view.
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