› 2d5b

X-ray diffraction
1.8Å resolution

Crystal Structure of Thermus Thermophilus Methionyl tRNA synthetase Y225F Mutant obtained in the presence of PEG6000

Released:
DOI: 10.2210/pdb2d5b/pdb
PDB entry 2d5b coloured by chain, front view.
PDB entry 2d5b coloured by chain, side view.
PDB entry 2d5b coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Konno M, Takeda R, Takasaka R, Mori Y, Ishii R, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 58 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23395 (Residues: 1-500; Coverage: 81%)
Gene names: TTHA1298, metG, metS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 50.14Å b: 82.25Å c: 121.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.242
Expression system: Escherichia coli BL21(DE3)

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