2cpu Summary

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SUBSITE MAPPING OF THE ACTIVE SITE OF HUMAN PANCREATIC ALPHA-AMYLASE USING SUBSTRATES, THE PHARMACOLOGICAL INHIBITOR ACARBOSE, AND AN ACTIVE SITE VARIANT

The structure was published by Brayer, G.D., Sidhu, G., Maurus, R., et al., Nguyen, N.T., Overall, C.M., and Withers, S.G., in 2000 in a paper entitled "Subsite mapping of the human pancreatic alpha-amylase active site through structural, kinetic, and mutagenesis techniques." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 1999.

The experimental data on which the structure is based was not deposited.

The PDB entry contains the structure of ALPHA-AMYLASE. This molecule has the UniProt identifier P04746 (AMYP_HUMAN)search. The sample contained 496 residues which is < 90% of the natural sequence. Out of 496 residues 496 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A ALPHA-AMYLASE P04746 (17-314) (AMYP_HUMAN)search ,
P04746 (316-511) (AMYP_HUMAN)search
Homo sapienssearch ,
Homo sapienssearch
100% ,
100%
496 100%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P04746 (17 - 314) ALPHA-AMYLASE Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P04746) Amylase, catalytic domainsearch, alpha-Amylases, C-terminal beta-sheet domainsearch Glycosidasessearch, Golgi alpha-mannosidase IIsearch PF00128: Alpha amylase, catalytic domainsearch, PF02806: Alpha amylase, C-terminal all-beta domainsearch

Chain ID Biological process (GO) Molecular function (GO)
A (P04746) carbohydrate metabolic processsearch catalytic activitysearch cation bindingsearch

Chain InterPro annotation
A Alpha amylasesearch Glycosyl hydrolase, family 13, catalytic domainsearch Alpha-amylase, C-terminal all betasearch Glycosyl hydrolase, family 13, subfamily, catalytic domainsearch Glycosyl hydrolase, family 13, all-betasearch Glycoside hydrolase, catalytic domainsearch Glycoside hydrolase, family 13search Glycoside hydrolase, superfamilysearch