› 2cn4

X-ray diffraction
2.3Å resolution

The crystal structure of the secreted dimeric form of the hemophore HasA reveals a domain swapping with an exchanged heme ligand

Released:
DOI: 10.2210/pdb2cn4/pdb
PDB entry 2cn4 coloured by chain, front view.
PDB entry 2cn4 coloured by chain, side view.
PDB entry 2cn4 coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
The crystal structure of the secreted dimeric form of the hemophore HasA reveals a domain swapping with an exchanged heme ligand.
Czjzek M, Létoffé S, Wandersman C, Delepierre M, Lecroisey A, Izadi-Pruneyre N
J Mol Biol 365 1176-86 (2007)
PMID: 17113104

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemophore HasA Chains: A, B
Molecule details ›
Chains: A, B
Length: 173 amino acids
Theoretical weight: 17.77 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54450 (Residues: 2-174; Coverage: 92%)
Gene name: hasA
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 47.08Å b: 62.32Å c: 56.82Å
α: 90° β: 105.34° γ: 90°
R-values:
R R work R free
0.172 0.169 0.224
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Type 1 secretion pathway - the hemolysin system and beyond.
Thomas et al. (2014)
5 more

2 mentions without citation

Linking in domain-swapped protein dimers.
Baiesi et al. (2016)
1 more