› 2cjw

X-ray diffraction
2.1Å resolution

Crystal structure of the small GTPase Gem (GemDNDCaM) in complex to Mg.GDP

Released:
DOI: 10.2210/pdb2cjw/pdb
PDB entry 2cjw coloured by chain, front view.
PDB entry 2cjw coloured by chain, side view.
PDB entry 2cjw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical and structural characterization of the gem GTPase.
Splingard A, Ménétrey J, Perderiset M, Cicolari J, Regazzoni K, Hamoudi F, Cabanié L, El Marjou A, Wells A, Houdusse A, de Gunzburg J
J Biol Chem 282 1905-15 (2007)
PMID: 17107948

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157157 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding protein GEM Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 22.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55040 (Residues: 70-261; Coverage: 65%)
Gene names: GEM, KIR
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
GTP-binding protein GEM Chain: B
Molecule details ›
Chain: B
Length: 192 amino acids
Theoretical weight: 22.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55040 (Residues: 70-261; Coverage: 65%)
Gene names: GEM, KIR
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
1 modified residue:
Ligand CAS 8 x CAS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P61
Unit cell:
a: 116.617Å b: 116.617Å c: 81.409Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.241
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
8 more

6 mentions without citation

Transplanting supersites of HIV-1 vulnerability.
Zhou et al. (2014)
5 more