2cjm Summary


Mechanism of CDK inhibition by active site phosphorylation: CDK2 Y15p T160p in complex with cyclin A structure

The structure was published by Welburn, J.P.I., Tucker, J., Johnson, T., et al., Willis, A., Noble, M.E.M., and Endicott, J.A., in 2007 in a paper entitled "How Tyrosine 15 Phosphorylation Inhibits the Activity of Cyclin-Dependent Kinase 2-Cyclin A." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.3 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely CELL DIVISION PROTEIN KINASE 2 and CYCLIN A2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CELL DIVISION PROTEIN KINASE 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 99% 298 99%
C CELL DIVISION PROTEIN KINASE 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 99% 298 99%
B CYCLIN A2 P20248 (175-432) (CCNA2_HUMAN)search Homo sapienssearch < 90% 258 99%
D CYCLIN A2 P20248 (175-432) (CCNA2_HUMAN)search Homo sapienssearch < 90% 258 99%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P24941 (1 - 298) CELL DIVISION PROTEIN KINASE 2 Homo sapiens
P20248 (175 - 432) CYCLIN A2 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P24941) Protein kinases, catalytic subunitsearch Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch
B, D Cyclinsearch Cyclin A; domain 1search Cyclin, N-terminal domainsearch, Cyclin, C-terminal domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (P24941) ATP bindingsearch protein kinase activitysearch transferase activitysearch protein bindingsearch cyclin-dependent protein serine/threonine kinase activitysearch metal ion bindingsearch protein serine/threonine kinase activitysearch nucleotide bindingsearch histone kinase activitysearch kinase activitysearch cyclin bindingsearch transferase activity, transferring phosphorus-containing groupssearch DNA replicationsearch G2/M transition of mitotic cell cyclesearch G1/S transition of mitotic cell cyclesearch meiotic cell cyclesearch protein phosphorylationsearch cell divisionsearch blood coagulationsearch regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch mitotic G1 DNA damage checkpointsearch cell cyclesearch regulation of gene silencingsearch cellular response to nitric oxidesearch positive regulation of transcription, DNA-templatedsearch meiotic nuclear divisionsearch DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestsearch anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processsearch positive regulation of cell proliferationsearch Ras protein signal transductionsearch centrosome duplicationsearch positive regulation of DNA-dependent DNA replication initiationsearch cellular response to DNA damage stimulussearch mitotic cell cyclesearch DNA repairsearch potassium ion transportsearch phosphorylationsearch mitotic nuclear divisionsearch histone phosphorylationsearch nucleoplasmsearch cytosolsearch nucleussearch chromosome, telomeric regionsearch centrosomesearch cytoplasmsearch cyclin-dependent protein kinase holoenzyme complexsearch Y chromosomesearch endosomesearch Cajal bodysearch cytoskeletonsearch X chromosomesearch condensed chromosomesearch transcription factor complexsearch microtubule organizing centersearch
B, D (P20248) protein kinase bindingsearch regulation of cyclin-dependent protein serine/threonine kinase activitysearch regulation of G2/M transition of mitotic cell cyclesearch regulation of cell cyclesearch nucleussearch

Chain InterPro annotation
A, C Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B, D Cyclin, C-terminal domainsearch Cyclin, N-terminalsearch Cyclin-likesearch Cyclin A/B/D/E/F/Osearch Cyclin A, chordatessearch