› 2ceq

X-ray diffraction
2.14Å resolution

Beta-glycosidase from Sulfolobus solfataricus in complex with glucoimidazole

Released:
DOI: 10.2210/pdb2ceq/pdb
PDB entry 2ceq coloured by chain, front view.
PDB entry 2ceq coloured by chain, side view.
PDB entry 2ceq coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structural, kinetic, and thermodynamic analysis of glucoimidazole-derived glycosidase inhibitors.
Gloster TM, Roberts S, Perugino G, Rossi M, Moracci M, Panday N, Terinek M, Vasella A, Davies GJ
Biochemistry 45 11879-84 (2006)
PMID: 17002288

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 56.76 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P22498 (Residues: 1-489; Coverage: 100%)
Gene names: SSO3019, lacS
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand ACT 5 x ACT
Ligand GIM 2 x GIM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3121
Unit cell:
a: 167.927Å b: 167.927Å c: 93.42Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.215
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

β-Glucosidases.
Ketudat Cairns et al. (2010)
4 more

5 mentions without citation

Application of the PM6 semi-empirical method to modeling proteins enhances docking accuracy of AutoDock.
Bikadi et al. (2009)
4 more