› 2cd7

X-ray diffraction
1.5Å resolution

Staphylococcus aureus pI258 arsenate reductase (ArsC) H62Q mutant

Released:
DOI: 10.2210/pdb2cd7/pdb
PDB entry 2cd7 coloured by chain, front view.
PDB entry 2cd7 coloured by chain, side view.
PDB entry 2cd7 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Interplay between ion binding and catalysis in the thioredoxin-coupled arsenate reductase family.
Roos G, Buts L, Van Belle K, Brosens E, Geerlings P, Loris R, Wyns L, Messens J
J Mol Biol 360 826-38 (2006)
PMID: 16797027

Function and Biology Details

Reaction catalysed: 
Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arsenate reductase Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.82 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A006 (Residues: 1-131; Coverage: 100%)
Gene name: arsC
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 33.662Å b: 33.226Å c: 100.601Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.218
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Understanding the pK(a) of redox cysteines: the key role of hydrogen bonding.
Roos et al. (2013)
2 more

1 mention without citation

Structural and functional mapping of ars gene cluster in Deinococcus indicus DR1.
Ranganathan et al. (2023)