2ca5

X-ray diffraction
2.1Å resolution

MxiH needle protein of Shigella Flexneri (monomeric form, residues 1- 78)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-141046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 3 secretion system needle filament protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 9.55 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A223 (Residues: 1-81; Coverage: 98%)
Gene names: CP0137, mxiH, sctF
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 183.203Å b: 28.123Å c: 27.723Å
α: 90° β: 96.47° γ: 90°
R-values:
R R work R free
0.196 0.194 not available
Expression system: Escherichia coli BL21(DE3)