› 2c9l

X-ray diffraction
2.25Å resolution

Structure of the Epstein-Barr virus ZEBRA protein

Released:
DOI: 10.2210/pdb2c9l/pdb
PDB entry 2c9l coloured by chain, front view.
PDB entry 2c9l coloured by chain, side view.
PDB entry 2c9l coloured by chain, top view.
Source organism: Human gammaherpesvirus 4
Primary publication:
Structural basis of lytic cycle activation by the Epstein-Barr virus ZEBRA protein.
Petosa C, Morand P, Baudin F, Moulin M, Artero JB, Müller CW
Mol Cell 21 565-72 (2006)
PMID: 16483937

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115143 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Lytic switch protein BZLF1 Chains: Y, Z
Molecule details ›
Chains: Y, Z
Length: 63 amino acids
Theoretical weight: 7.41 KDa
Source organism: Human gammaherpesvirus 4
Expression system: Escherichia coli
UniProt:
  • Canonical: P03206 (Residues: 175-236; Coverage: 25%)
Gene name: BZLF1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
5'-D(*AP*AP*GP*CP*AP*CP*TP*GP*AP*CP *TP*CP*AP*TP*GP*AP*AP*GP*T)-3' Chain: A
Molecule details ›
Chain: A
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Human gammaherpesvirus 4
Expression system: Not provided
5'-D(*AP*CP*TP*TP*CP*AP*CP*TP*GP*AP *GP*TP*CP*AP*GP*TP*GP*CP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: Human gammaherpesvirus 4
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: C2
Unit cell:
a: 94.17Å b: 26.52Å c: 98.09Å
α: 90° β: 103.95° γ: 90°
R-values:
R R work R free
0.232 0.232 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

The CCAAT/enhancer (C/EBP) family of basic-leucine zipper (bZIP) transcription factors is a multifaceted highly-regulated system for gene regulation.
Tsukada et al. (2011)
8 more

13 mentions without citation

Methylation-dependent binding of the epstein-barr virus BZLF1 protein to viral promoters.
Dickerson et al. (2009)
12 more