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EBI PDBe PDBeView

PDBe Entry: 2c7p view

HHAI DNA METHYLTRANSFERASE COMPLEX WITH OLIGONUCLEOTIDE CONTAINING 2-AMINOPURINE OPPOSITE TO THE TARGET BASE (GCGC:GMPC) AND SAH
Summary
Header TRANSFERASEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 1.7 Å, R-factor: 19.6%, Free R-factor: 22.0%, Spacegroup: H 3 2
Released 14/12/2005, deposition: 25/11/2005, last revision: 24/02/2009
Authors Daujotyte, D.search; Grazulis, S.search
Primary citation Time-Resolved Fluorescence of 2-Aminopurine as a Probe of Base Flipping in M.HhaI-DNA Complexes.
NUCLEIC ACIDS RES.search vol:33, pag:6953 (2005) [PubMed ID 16340006 ]search
Keywords DNA METHYLTRANSFERASEsearch, METHYLTRANSFERASEsearch, BASE FLIPPINGsearch, RESTRICTION SYSTEMsearch, TRANSFERASEsearch
EC 2.1.1.37 ExPASy BRENDA search (A)
Organism Haemophilus haemolyticus 726search(A)
UniProt Modification methylase HhaI (EC 2.1.1.37) (M.HhaI) (Cytosine-specific methyltransferase HhaI) P05102search (A)
Solvent A, C, D
Related entries 10mh, 1fjx, 1hmy, 1m0e, 1mht, 1skm, 1svu, 2hmy, 3mht, 4mht, 5mht, 6mht, 7mht, 8mht, 9mht, 2c7o, 2c7q, 2c7r
Polymers
Id Name Type UniProt Residues Observed
A MODIFICATION METHYLASE HHAI Protein P05102 (MTH1_HAEPH)search
 327 100%
C 5'-D(*G*GP*AP*TP*GP*(5CM*A1P)*CP*TP*GP*AP*C)-3' DNA
12 100%
D 5'-D(*G*TP*CP*AP*GP*CP*GP*CP*AP*TP*CP*C)-3' DNA
12 100%
Heterogens
Id Name Ligands
A S-ADENOSYL-L-HOMOCYSTEINE SAH search
A SULFATE ION SO4 search
A 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID EPE search
A CITRIC ACID CIT search
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