› 2c6f

X-ray diffraction
3.01Å resolution

Structure of human somatic angiontensin-I converting enzyme N domain

Released:
DOI: 10.2210/pdb2c6f/pdb
PDB entry 2c6f coloured by chain, front view.
PDB entry 2c6f coloured by chain, side view.
PDB entry 2c6f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the N domain of human somatic angiotensin I-converting enzyme provides a structural basis for domain-specific inhibitor design.
Corradi HR, Schwager SL, Nchinda AT, Sturrock ED, Acharya KR
J Mol Biol 357 964-74 (2006)
PMID: 16476442

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 612 amino acids
Theoretical weight: 70.54 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-641; Coverage: 48%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand NAG 5 x NAG
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand ACT 3 x ACT
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2221
Unit cell:
a: 101.12Å b: 211.32Å c: 171.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.273
Expression system: Cricetulus griseus

Quick links

Citations

11 review citations

A modern understanding of the traditional and nontraditional biological functions of angiotensin-converting enzyme.
Bernstein et al. (2013)
10 more

8 mentions without citation

PharmGKB: understanding the effects of individual genetic variants.
Sangkuhl et al. (2008)
7 more