› 2bv5

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF THE HUMAN PROTEIN TYROSINE PHOSPHATASE PTPN5 AT 1.8A RESOLUTION

Released:
DOI: 10.2210/pdb2bv5/pdb
PDB entry 2bv5 coloured by chain, front view.
PDB entry 2bv5 coloured by chain, side view.
PDB entry 2bv5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures and inhibitor identification for PTPN5, PTPRR and PTPN7: a family of human MAPK-specific protein tyrosine phosphatases.
Eswaran J, von Kries JP, Marsden B, Longman E, Debreczeni JE, Ugochukwu E, Turnbull A, Lee WH, Knapp S, Barr AJ
Biochem J 395 483-91 (2006)
PMID: 16441242

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 5 Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54829 (Residues: 280-561; Coverage: 50%)
Gene name: PTPN5
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand SCY 1 x SCY

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 39.957Å b: 64.01Å c: 136.149Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Therapeutic implications for striatal-enriched protein tyrosine phosphatase (STEP) in neuropsychiatric disorders.
Goebel-Goody et al. (2012)
10 more

11 mentions without citation

Protein tyrosine phosphatases: structure, function, and implication in human disease.
Tautz et al. (2013)
10 more