› 2bto

X-ray diffraction
2.5Å resolution

Structure of BtubA from Prosthecobacter dejongeii

Released:
DOI: 10.2210/pdb2bto/pdb
PDB entry 2bto coloured by chain, front view.
PDB entry 2bto coloured by chain, side view.
PDB entry 2bto coloured by chain, top view.
Source organisms:
Primary publication:
Structure of bacterial tubulin BtubA/B: evidence for horizontal gene transfer.
Schlieper D, Oliva MA, Andreu JM, Löwe J
Proc Natl Acad Sci U S A 102 9170-5 (2005)
PMID: 15967998

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141964 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin/FtsZ GTPase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 51.3 KDa
Source organism: Prosthecobacter dejongeii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GCC5 (Residues: 1-473; Coverage: 100%)
Gene name: btubA
Sequence domains:
Structure domains:
Thioredoxin 1 Chain: T
Molecule details ›
Chain: T
Length: 108 amino acids
Theoretical weight: 11.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P321
Unit cell:
a: 180.539Å b: 180.539Å c: 84.229Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.235
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Horizontal gene transfer in eukaryotic evolution.
Keeling et al. (2008)
40 more

1 mention without citation

Structure of bacterial tubulin BtubA/B: evidence for horizontal gene transfer.
Schlieper et al. (2005)