2br2

X-ray diffraction
2.8Å resolution

RNase PH core of the archaeal exosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194120 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Exosome complex component Rrp42 Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Length: 275 amino acids
Theoretical weight: 30.23 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UXC0 (Residues: 1-275; Coverage: 100%)
Gene names: C20_023, SSO0732, rrp42
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component Rrp41 Chains: B, D, F, H, J, L, N, P, R, T, V, X
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T, V, X
Length: 248 amino acids
Theoretical weight: 27.61 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UXC2 (Residues: 1-248; Coverage: 100%)
Gene names: SSO0735, rrp41
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 206.88Å b: 212.72Å c: 434.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.237
Expression system: Escherichia coli BL21(DE3)