Function and Biology Details
Reaction catalysed:
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO(2)
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
2-oxoisovalerate dehydrogenase (preferred)
PDBe Complex ID:
PDB-CPX-140547 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2-oxoisovalerate dehydrogenase subunit alpha
Molecule details ›
Chains: A, C, E, G
Length: 410 amino acids
Theoretical weight: 45.32 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Structure domains: Rossmann fold
Length: 410 amino acids
Theoretical weight: 45.32 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P09060 (Residues: 1-410; Coverage: 100%)
Sequence domains:
Structure domains: Rossmann fold
2-oxoisovalerate dehydrogenase subunit beta
Molecule details ›
Chains: B, D, F, H
Length: 339 amino acids
Theoretical weight: 37.17 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Structure domains:
Length: 339 amino acids
Theoretical weight: 37.17 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P09061 (Residues: 1-339; Coverage: 100%)
Sequence domains:
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-1
Spacegroup:
P6122
Expression system: Escherichia coli BL21(DE3)
