› 2boz

X-ray diffraction
2.4Å resolution

Photosynthetic Reaction Center Mutant With Gly M203 Replaced With Leu

Released:
DOI: 10.2210/pdb2boz/pdb
PDB entry 2boz coloured by chain, front view.
PDB entry 2boz coloured by chain, side view.
PDB entry 2boz coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Strong effects of an individual water molecule on the rate of light-driven charge separation in the Rhodobacter sphaeroides reaction center.
Potter JA, Fyfe PK, Frolov D, Wakeham MC, van Grondelle R, Robert B, Jones MR
J Biol Chem 280 27155-64 (2005)
PMID: 15908429

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 307 amino acids
Theoretical weight: 34.45 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like

Ligands and Environments

8 bound ligands:
Ligand LDA 4 x LDA
Ligand D10 3 x D10
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand U10 2 x U10
Ligand FE 1 x FE
Ligand PO4 2 x PO4
Ligand SPN 1 x SPN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P3121
Unit cell:
a: 138.613Å b: 138.613Å c: 185.274Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.174 0.198
Expression system: Cereibacter sphaeroides

Quick links

Citations

2 review citations

Solid-state NMR of nanomachines involved in photosynthetic energy conversion.
Alia et al. (2013)
1 more

2 mentions without citation

'Double water exclusion': a hypothesis refining the O-ring theory for the hot spots at protein interfaces.
Li et al. (2009)
1 more