› 2bm1

X-ray diffraction
2.6Å resolution

Ribosomal elongation factor G (EF-G) Fusidic acid resistant mutant G16V

Released:
DOI: 10.2210/pdb2bm1/pdb
PDB entry 2bm1 coloured by chain, front view.
PDB entry 2bm1 coloured by chain, side view.
PDB entry 2bm1 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Structural insights into fusidic acid resistance and sensitivity in EF-G.
Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT
J Mol Biol 348 939-49 (2005)
PMID: 15843024

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elongation factor G Chain: A
Molecule details ›
Chain: A
Length: 691 amino acids
Theoretical weight: 77.02 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13551 (Residues: 1-691; Coverage: 100%)
Gene names: fus, fusA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 76.3Å b: 89.7Å c: 114.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Dumb and dumber--the potential waste of a useful antistaphylococcal agent: emerging fusidic acid resistance in Staphylococcus aureus.
Howden et al. (2006)
3 more

1 mention without citation

Sensory distinction between H-2b and H-2bm1 mutant mice.
Yamazaki et al. (1983)