› 2bl7

X-ray diffraction
2.2Å resolution

1.6 Angstrom crystal structure of EntA-im: a bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin, enterocin A

Released:
DOI: 10.2210/pdb2bl7/pdb
PDB entry 2bl7 coloured by chain, front view.
PDB entry 2bl7 coloured by chain, side view.
PDB entry 2bl7 coloured by chain, top view.
Source organism: Enterococcus faecium
Primary publication:
1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A.
Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J
J Biol Chem 280 19045-50 (2005)
PMID: 15753083

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriocin immunity protein Chain: A
Molecule details ›
Chain: A
Length: 103 amino acids
Theoretical weight: 12.41 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47785 (Residues: 1-103; Coverage: 100%)
Gene names: CUN04_07130, DTX73_06505, GBM44_11695, GBM73_12235, KYX88_02275, M3X98_13385, SAP083A_011, entI, orf2
Sequence domains: Enterocin A Immunity
Structure domains: Ta0600-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 75.8Å b: 75.8Å c: 36.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.21 0.261
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The continuing story of class IIa bacteriocins.
Drider et al. (2006)
3 more

2 mentions without citation

Perspectives in Searching Antimicrobial Peptides (AMPs) Produced by the Microbiota.
Gallardo-Becerra et al. (2023)
1 more