2bif Summary

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6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE H256A MUTANT WITH F6P IN PHOSPHATASE ACTIVE SITE

The structure was published by Yuen, M.H., Mizuguchi, H., Lee, Y.H., Cook, P.F., Uyeda, K., and Hasemann, C.A., in 1999 in a paper entitled "Crystal structure of the H256A mutant of rat testis fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase. Fructose 6-phosphate in the active site leads to mechanisms for both mutant and wild type bisphosphatase activities." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.4 Å and deposited in 1998.

The experimental data on which the structure is based was also deposited.

This PDB entry contains multiple copies of the structure of PROTEIN (6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE).

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms homodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A PROTEIN (6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE) P25114 (1-469) (F264_RAT)search Rattus norvegicussearch 100% 469 92%
B PROTEIN (6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE) P25114 (1-469) (F264_RAT)search Rattus norvegicussearch 100% 469 92%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P25114 (1 - 469) PROTEIN (6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE) Rattus norvegicus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (P25114) 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, kinase domainsearch, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, phosphatase domainsearch P-loop containing nucleotide triphosphate hydrolasessearch, Phosphoglycerate mutase-likesearch PF00300: Histidine phosphatase superfamily (branch 1)search, PF01591: 6-phosphofructo-2-kinasesearch

Chain ID Molecular function (GO) Biological process (GO)
A, B (P25114) catalytic activitysearch 6-phosphofructo-2-kinase activitysearch fructose-2,6-bisphosphate 2-phosphatase activitysearch ATP bindingsearch nucleotide bindingsearch transferase activitysearch hydrolase activitysearch kinase activitysearch fructose 2,6-bisphosphate metabolic processsearch fructose metabolic processsearch phosphorylationsearch metabolic processsearch

Chain InterPro annotation
A, B Fructose-2,6-bisphosphatasesearch Histidine phosphatase superfamily, clade-1search 6-phosphofructo-2-kinasesearch Bifunctional 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphate 2-phosphatasesearch P-loop containing nucleoside triphosphate hydrolasesearch