› 2bh3

X-ray diffraction
2.4Å resolution

Zn substituted E. coli Aminopeptidase P in complex with product

Released:
DOI: 10.2210/pdb2bh3/pdb
PDB entry 2bh3 coloured by chain, front view.
PDB entry 2bh3 coloured by chain, side view.
PDB entry 2bh3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and functional implications of metal ion selection in aminopeptidase P, a metalloprotease with a dinuclear metal center.
Graham SC, Bond CS, Freeman HC, Guss JM
Biochemistry 44 13820-36 (2005)
PMID: 16229471

Function and Biology Details

Reaction catalysed: 
Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xaa-Pro aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 49.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P15034 (Residues: 2-441; Coverage: 100%)
Gene names: JW2876, b2908, pepP
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand PRO 1 x PRO
Ligand LEU 1 x LEU
Ligand ZN 4 x ZN
Ligand MG 1 x MG
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200H
Spacegroup: I4122
Unit cell:
a: 138.012Å b: 138.012Å c: 230.734Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.201
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metallostasis and amyloid β-degrading enzymes.
Grasso et al. (2012)
2 more

2 mentions without citation

Complexes of hydrophilic triphenylphosphines modified with gem-bis(phosphonate) moiety. An unusual simultaneous cis and trans arrangements in the Pt(II) dinuclear complex.
Drahos et al. (2009)
1 more