2bec Summary


Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation

The structure was published by Ben Ammar, Y., Takeda, S., Hisamitsu, T., Mori, H., and Wakabayashi, S., in 2006 in a paper entitled "Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.7 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calcineurin B homologous protein 2 and Sodium/hydrogen exchanger 1.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calcineurin B homologous protein 2 O43745 (1-196) (CHP2_HUMAN)search Homo sapienssearch 100% 202 89%
B Sodium/hydrogen exchanger 1 P19634 (503-545) (SL9A1_HUMAN)search Homo sapienssearch < 90% 43 58%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
O43745 (1 - 196) Calcineurin B homologous protein 2 Homo sapiens
P19634 (503 - 545) Sodium/hydrogen exchanger 1 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (O43745) EF-handsearch PF00036: EF handsearch, PF13405: EF-hand domainsearch
B Sodium/hydrogen exchanger familysearch

Chain ID Cellular component (GO) Biological process (GO) Molecular function (GO)
A (O43745) plasma membranesearch nucleussearch membranesearch cytoplasmsearch transportsearch positive regulation of calcineurin-NFAT signaling cascadesearch protein transportsearch cellular response to calcium ionsearch positive regulation of protein import into nucleussearch positive regulation of transcription from RNA polymerase II promotersearch positive regulation of phosphatase activitysearch positive regulation of cell proliferationsearch protein bindingsearch calcium ion bindingsearch metal ion bindingsearch
B (P19634) integral component of membranesearch cation transportsearch solute:proton antiporter activitysearch

Chain InterPro annotation
A EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch
B Cation/H+ exchanger, CPA1 familysearch