› 2bdm

X-ray diffraction
2.3Å resolution

Structure of Cytochrome P450 2B4 with Bound Bifonazole

Released:
DOI: 10.2210/pdb2bdm/pdb
PDB entry 2bdm coloured by chain, front view.
PDB entry 2bdm coloured by chain, side view.
PDB entry 2bdm coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction.
Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD
J Biol Chem 281 5973-81 (2006)
PMID: 16373351

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2B4 Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 54.17 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00178 (Residues: 20-491; Coverage: 96%)
Gene name: CYP2B4
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand TMI 3 x TMI
Ligand CM5 4 x CM5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P6122
Unit cell:
a: 203.21Å b: 203.21Å c: 103.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.196 0.215
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily.
Munro et al. (2007)
19 more

22 mentions without citation

Cooperative properties of cytochromes P450.
Denisov et al. (2009)
21 more