› 2bcu

X-ray diffraction
2.2Å resolution

DNA polymerase lambda in complex with a DNA duplex containing an unpaired Damp and a T:T mismatch

Released:
DOI: 10.2210/pdb2bcu/pdb
PDB entry 2bcu coloured by chain, front view.
PDB entry 2bcu coloured by chain, side view.
PDB entry 2bcu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of strand misalignment during DNA synthesis by a human DNA polymerase.
Garcia-Diaz M, Bebenek K, Krahn JM, Pedersen LC, Kunkel TA
Cell 124 331-42 (2006)
PMID: 16439207

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115110 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase lambda Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 37.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGP5 (Residues: 242-575; Coverage: 58%)
Gene name: POLL
Sequence domains:
Structure domains:
5'-D(P*GP*CP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*AP*GP*TP*TP*CP*G)-3' Chain: P
Molecule details ›
Chain: P
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*GP*GP*CP*CP*GP*AP*TP*AP*CP*TP*G)-3' Chain: T
Molecule details ›
Chain: T
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 3 x NA
Ligand PPV 1 x PPV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 55.998Å b: 62.667Å c: 140.494Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.247
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Mechanism of a genetic glissando: structural biology of indel mutations.
Garcia-Diaz et al. (2006)
8 more

2 mentions without citation

The X family portrait: structural insights into biological functions of X family polymerases.
Moon et al. (2007)
1 more