2b3t Summary

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Molecular basis for bacterial class 1 release factor methylation by PrmC

The structure was published by Graille, M., Heurgue-Hamard, V., Champ, S., et al., Ulryck, N., van Tilbeurgh, H., and Buckingham, R.H., in 2005 in a paper entitled "Molecular basis for bacterial class I release factor methylation by PrmC" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.1 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Protein methyltransferase hemK and Peptide chain release factor 1.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Protein methyltransferase hemK P0ACC1 (1-276) (PRMC_ECOLI)search Escherichia coli K-12search 100% 276 100%
B Peptide chain release factor 1 P0A7I0 (1-356) (RF1_ECOLI)search Escherichia coli K-12search 99% 360 83%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P0ACC1 (1 - 276) Protein methyltransferase hemK Escherichia coli
P0A7I0 (1 - 356) Peptide chain release factor 1 Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P0ACC1) N5-glutamine methyltransferase, HemKsearch DNA helicase RuvA subunit, C-terminal domainsearch, Vaccinia Virus protein VP39search PF13847: Methyltransferase domainsearch
B (P0A7I0) Release factorsearch PF00472: RF-1 domainsearch, PF03462: PCRF domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P0ACC1) methyltransferase activitysearch transferase activitysearch protein-glutamine N-methyltransferase activitysearch S-adenosylmethionine-dependent methyltransferase activitysearch nucleic acid bindingsearch protein methyltransferase activitysearch methylationsearch peptidyl-glutamine methylationsearch regulation of gene expressionsearch protein methylationsearch translational terminationsearch
B (P0A7I0) translation release factor activity, codon specificsearch ribosome bindingsearch translation release factor activitysearch translational terminationsearch translationsearch cytoplasmsearch

Chain InterPro annotation
A DNA methylase, N-6 adenine-specific, conserved sitesearch Modification methylase HemKsearch Protein-(glutamine-N5) methyltransferase, release factor-specificsearch Methyltransferase domainsearch S-adenosyl-L-methionine-dependent methyltransferasesearch
B Peptide chain release factor class I/class IIsearch Peptide chain release factor 1search Peptide chain release factorsearch Double-stranded RNA-binding domainsearch