› 2ay6

X-ray diffraction
2.2Å resolution

AROMATIC AMINO ACID AMINOTRANSFERASE WITH 3-INDOLEBUTYRIC ACID

Released:
DOI: 10.2210/pdb2ay6/pdb
PDB entry 2ay6 coloured by chain, front view.
PDB entry 2ay6 coloured by chain, side view.
PDB entry 2ay6 coloured by chain, top view.
Source organism: Paracoccus denitrificans
Primary publication:
The active site of Paracoccus denitrificans aromatic amino acid aminotransferase has contrary properties: flexibility and rigidity.
Okamoto A, Ishii S, Hirotsu K, Kagamiyama H
Biochemistry 38 1176-84 (1999)
PMID: 9930977

Function and Biology Details

Reaction catalysed: 
An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aromatic-amino-acid aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 42.78 KDa
Source organism: Paracoccus denitrificans
Expression system: Escherichia coli
UniProt:
  • Canonical: P95468 (Residues: 1-394; Coverage: 100%)
Gene name: tyrB
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand 3IB 1 x 3IB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 124.01Å b: 121.78Å c: 55.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.258
Expression system: Escherichia coli

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Citations

4 review citations

Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
Eliot et al. (2004)
3 more