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Solution NMR

THE SOLUTION STRUCTURE OF OXIDIZED RAT MICROSOMAL CYTOCHROME B5, NMR, 21 STRUCTURES

Released:
DOI: 10.2210/pdb2axx/pdb
PDB entry 2axx coloured by chain, ensemble of 21 models, front view.
PDB entry 2axx coloured by chain, ensemble of 21 models, side view.
PDB entry 2axx coloured by chain, ensemble of 21 models, top view.
Source organism: Rattus norvegicus
Primary publication:
The solution structure of oxidized rat microsomal cytochrome b5.
Arnesano F, Banci L, Bertini I, Felli IC
Biochemistry 37 173-84 (1998)
PMID: 9425037

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132305 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.81 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00173 (Residues: 6-99; Coverage: 70%)
Gene names: Cyb5, Cyb5a
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE MOLECULAR DYNAMICS SIMULATED ANNEALING, RESTRAINED ENERGY MINIMIZATION; PSEUDOCONTACT SHIFTS WERE USED IN THE CALCULATION AND IN THE MINIMIZATION AS FURTHER NON-CLASSICAL CONSTRAINTS
Expression system: Escherichia coli

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Citations

3 review citations

The use of chemical shifts and their anisotropies in biomolecular structure determination.
Case DA. (1998)
2 more