› 2awz

X-ray diffraction
2.15Å resolution

Hepatitis C Virus NS5b RNA Polymerase in complex with a covalent inhibitor (5h)

Released:
DOI: 10.2210/pdb2awz/pdb
PDB entry 2awz coloured by chain, front view.
PDB entry 2awz coloured by chain, side view.
PDB entry 2awz coloured by chain, top view.
Source organism: Hepacivirus C
Primary publication:
SAR and mode of action of novel non-nucleoside inhibitors of hepatitis C NS5b RNA polymerase.
Powers JP, Piper DE, Li Y, Mayorga V, Anzola J, Chen JM, Jaen JC, Lee G, Liu J, Peterson MG, Tonn GR, Ye Q, Walker NP, Wang Z
J Med Chem 49 1034-46 (2006)
PMID: 16451069

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 580 amino acids
Theoretical weight: 64.64 KDa
Source organism: Hepacivirus C
Expression system: Escherichia coli
UniProt:
  • Canonical: P26663 (Residues: 2420-2989; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand 5H 2 x 5H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 87.088Å b: 87.022Å c: 162.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.205 0.243
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure-function relationships among RNA-dependent RNA polymerases.
Ng et al. (2008)
7 more

1 mention without citation

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)