› 2atm

X-ray diffraction
2Å resolution

Crystal structure of the recombinant allergen Ves v 2

Released:
DOI: 10.2210/pdb2atm/pdb
PDB entry 2atm coloured by chain, front view.
PDB entry 2atm coloured by chain, side view.
PDB entry 2atm coloured by chain, top view.
Source organism: Vespula vulgaris
Primary publication:
Structure of recombinant Ves v 2 at 2.0 Angstrom resolution: structural analysis of an allergenic hyaluronidase from wasp venom.
Skov LK, Seppälä U, Coen JJ, Crickmore N, King TP, Monsalve R, Kastrup JS, Spangfort MD, Gajhede M
Acta Crystallogr D Biol Crystallogr 62 595-604 (2006)
PMID: 16699186

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hyaluronidase A Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 39 KDa
Source organism: Vespula vulgaris
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P49370 (Residues: 1-331; Coverage: 100%)
Sequence domains: Hyaluronidase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand MES 1 x MES
1 modified residue:
Ligand SME 1 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 42.674Å b: 95.056Å c: 100.326Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.215
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Bee, wasp and ant venomics pave the way for a component-resolved diagnosis of sting allergy.
de Graaf et al. (2009)
12 more

10 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
9 more