2ass Summary


Crystal structure of the Skp1-Skp2-Cks1 complex

The structure was published by Hao, B., Zheng, N., Schulman, B.A., et al., Miller, J.J., Pagano, M., and Pavletich, N.P., in 2005 in a paper entitled "Structural Basis of the Cks1-Dependent Recognition of p27(Kip1) by the SCF(Skp2) Ubiquitin Ligase." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.0 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely S-phase kinase-associated protein 1A, S-phase kinase-associated protein 2, and Cyclin-dependent kinases regulatory subunit 1.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A S-phase kinase-associated protein 1A P63208 (2-160) (SKP1_HUMAN)search Homo sapienssearch 98% 159 89%
B S-phase kinase-associated protein 2 Q13309 (89-424) (SKP2_HUMAN)search Homo sapienssearch < 90% 336 96%
C Cyclin-dependent kinases regulatory subunit 1 P61024 (5-73) (CKS1_HUMAN)search Homo sapienssearch 100% 69 100%

This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P63208 (2 - 160) S-phase kinase-associated protein 1A Homo sapiens
Q13309 (89 - 424) S-phase kinase-associated protein 2 Homo sapiens
P61024 (5 - 73) Cyclin-dependent kinases regulatory subunit 1 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P63208) Potassium Channel Kv1.1; Chain Asearch PF01466: Skp1 family, dimerisation domainsearch, PF03931: Skp1 family, tetramerisation domainsearch
B F-box domainsearch, Cyclin A/CDK2-associated p19, Skp2search Ribonuclease Inhibitorsearch F-box-likesearch
C (P61024) Cell cycle regulatory proteinssearch Cyclin-Dependent Kinase Subunit Type 2search PF01111: Cyclin-dependent kinase regulatory subunitsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A (P63208) protein bindingsearch ubiquitin-protein transferase activitysearch nucleoplasmsearch cytosolsearch Cul7-RING ubiquitin ligase complexsearch SCF ubiquitin ligase complexsearch PcG protein complexsearch extracellular vesicular exosomesearch SCF-dependent proteasomal ubiquitin-dependent protein catabolic processsearch mitotic cell cyclesearch histone H2A monoubiquitinationsearch ubiquitin-dependent protein catabolic processsearch anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processsearch protein ubiquitinationsearch Notch signaling pathwaysearch G1/S transition of mitotic cell cyclesearch G2/M transition of mitotic cell cyclesearch regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch viral processsearch
C (P61024) cyclin-dependent protein serine/threonine kinase regulator activitysearch cell cyclesearch

Chain InterPro annotation
A SKP1 componentsearch BTB/POZ foldsearch SKP1 component, dimerisationsearch SKP1 component, POZ domainsearch E3 ubiquitin ligase, SCF complex, Skp subunitsearch
B F-box domainsearch
C Cyclin-dependent kinase, regulatory subunitsearch