› 2ae8

X-ray diffraction
2.01Å resolution

Crystal Structure of Imidazoleglycerol-phosphate Dehydratase from Staphylococcus aureus subsp. aureus N315

Released:
DOI: 10.2210/pdb2ae8/pdb
PDB entry 2ae8 coloured by chain, front view.
PDB entry 2ae8 coloured by chain, side view.
PDB entry 2ae8 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus N315
Entry authors: Kim Y, Quartey P, Holzle D, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazoleglycerol-phosphate dehydratase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 221 amino acids
Theoretical weight: 24.85 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P64373 (Residues: 1-192; Coverage: 100%)
Gene names: SA2468, hisB
Sequence domains: Imidazoleglycerol-phosphate dehydratase
Structure domains: Imidazole glycerol phosphate dehydratase; domain 1

Ligands and Environments

1 bound ligand:
Ligand MG 16 x MG
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4
Unit cell:
a: 129.247Å b: 129.247Å c: 180.143Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.226
Expression system: Escherichia coli BL21(DE3)

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