› 2aat

X-ray diffraction
2.8Å resolution

2.8-ANGSTROMS-RESOLUTION CRYSTAL STRUCTURE OF AN ACTIVE-SITE MUTANT OF ASPARTATE AMINOTRANSFERASE FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb2aat/pdb
PDB entry 2aat coloured by chain, front view.
PDB entry 2aat coloured by chain, side view.
PDB entry 2aat coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
2.8-A-resolution crystal structure of an active-site mutant of aspartate aminotransferase from Escherichia coli.
Smith DL, Almo SC, Toney MD, Ringe D
Biochemistry 28 8161-7 (1989)
PMID: 2513875

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00509 (Residues: 1-396; Coverage: 100%)
Gene names: JW0911, aspC, b0928
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 1 x PMP
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 156.8Å b: 86.74Å c: 79.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 not available not available
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Principles of protein-protein interactions.
Jones et al. (1996)
3 more

3 mentions without citation

Crystal structure of Bacillus subtilis GabR, an autorepressor and transcriptional activator of gabT.
Edayathumangalam et al. (2013)
2 more