› 2a8c

X-ray diffraction
2.3Å resolution

Haemophilus influenzae beta-carbonic anhydrase

Released:
DOI: 10.2210/pdb2a8c/pdb
PDB entry 2a8c coloured by chain, front view.
PDB entry 2a8c coloured by chain, side view.
PDB entry 2a8c coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase.
Cronk JD, Rowlett RS, Zhang KY, Tu C, Endrizzi JA, Lee J, Gareiss PC, Preiss JR
Biochemistry 45 4351-61 (2006)
PMID: 16584170

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 229 amino acids
Theoretical weight: 26.29 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45148 (Residues: 1-229; Coverage: 100%)
Gene names: HI_1301, can
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 232.655Å b: 144.726Å c: 52.408Å
α: 90° β: 93.97° γ: 90°
R-values:
R R work R free
0.215 0.215 0.259
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure-based drug discovery of carbonic anhydrase inhibitors.
Supuran CT. (2012)
8 more

3 mentions without citation

An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii.
Aspatwar et al. (2018)
2 more