› 2a6a

X-ray diffraction
2.5Å resolution

Crystal structure of Glycoprotein endopeptidase (tm0874) from THERMOTOGA MARITIMA at 2.50 A resolution

Released:
DOI: 10.2210/pdb2a6a/pdb
PDB entry 2a6a coloured by chain, front view.
PDB entry 2a6a coloured by chain, side view.
PDB entry 2a6a coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structure of an essential bacterial protein YeaZ (TM0874) from Thermotoga maritima at 2.5 Å resolution.
Xu Q, McMullan D, Jaroszewski L, Krishna SS, Elsliger MA, Yeh AP, Abdubek P, Astakhova T, Axelrod HL, Carlton D, Chiu HJ, Clayton T, Duan L, Feuerhelm J, Grant J, Han GW, Jin KK, Klock HE, Knuth MW, Miller MD, Morse AT, Nigoghossian E, Okach L, Oommachen S, Paulsen J, Reyes R, Rife CL, van den Bedem H, Hodgson KO, Wooley J, Deacon AM, Godzik A, Lesley SA, Wilson IA
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 1230-6 (2010)
PMID: 20944216

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-194652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 24.62 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZX7 (Residues: 1-206; Coverage: 100%)
Gene names: TM_0874, Tmari_0876, tsaB
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UNL 2 x UNL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C222
Unit cell:
a: 93.272Å b: 217.112Å c: 51.953Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Conservation and Diversification of tRNA t6A-Modifying Enzymes across the Three Domains of Life.
Su et al. (2022)
1 more

10 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
9 more