2a3v

X-ray diffraction
2.8Å resolution

Structural basis for broad DNA-specificity in integron recombination

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-115059 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Site-specific recombinase IntIA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 320 amino acids
Theoretical weight: 37.5 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: O68847 (Residues: 1-320; Coverage: 100%)
Gene names: D6U24_18955, ERS013201_03858, F0M16_21740, FLM02_18510, intI4, intIA, xerD_2
Sequence domains:
Structure domains:
DNA (31-MER) Chains: E, G
Molecule details ›
Chains: E, G
Length: 40 nucleotides
Theoretical weight: 12.33 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Not provided
DNA (34-MER) Chains: F, H
Molecule details ›
Chains: F, H
Length: 43 nucleotides
Theoretical weight: 13.21 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 149.9Å b: 170.2Å c: 209.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.262
Expression systems:
  • Escherichia coli
  • Not provided