› 2a2a

X-ray diffraction
1.47Å resolution

High-resolution crystallographic analysis of the autoinhibited conformation of a human death-associated protein kinase

Released:
DOI: 10.2210/pdb2a2a/pdb
PDB entry 2a2a coloured by chain, front view.
PDB entry 2a2a coloured by chain, side view.
PDB entry 2a2a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites.
Simon B, Huart AS, Temmerman K, Vahokoski J, Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
Structure 24 851-61 (2016)
PMID: 27133022

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193792 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 321 amino acids
Theoretical weight: 37.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UIK4 (Residues: 11-330; Coverage: 87%)
Gene name: DAPK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 8 x NA
Ligand CL 3 x CL
Ligand DTT 4 x DTT
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P1
Unit cell:
a: 55.3Å b: 60.65Å c: 98.71Å
α: 92.16° β: 103.45° γ: 94.25°
R-values:
R R work R free
0.15 0.15 0.207
Expression system: Escherichia coli

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Citations

8 review citations

Crosstalk of ER stress-mediated autophagy and ER-phagy: Involvement of UPR and the core autophagy machinery.
Song et al. (2018)
7 more

6 mentions without citation

Synthesis and identification of new 4-arylidene curcumin analogues as potential anticancer agents targeting nuclear factor-κB signaling pathway.
Qiu et al. (2010)
5 more