› 2a14

X-ray diffraction
1.7Å resolution

Crystal Structure of Human Indolethylamine N-methyltransferase with SAH

Released:
DOI: 10.2210/pdb2a14/pdb
PDB entry 2a14 coloured by chain, front view.
PDB entry 2a14 coloured by chain, side view.
PDB entry 2a14 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Dong A, Wu H, Zeng H, Loppnau P, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + an amine = S-adenosyl-L-homocysteine + a methylated amine
S-adenosyl-L-methionine + dimethyl sulfide = S-adenosyl-L-homocysteine + trimethylsulfonium
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indolethylamine N-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 28.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95050 (Residues: 1-263; Coverage: 100%)
Gene name: INMT
Sequence domains: NNMT/PNMT/TEMT family
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: C2221
Unit cell:
a: 86.407Å b: 117.461Å c: 66.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.197
Expression system: Escherichia coli BL21(DE3)

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