2a0y Summary


Structure of human purine nucleoside phosphorylase H257D mutant

The structure was published by Murkin, A.S., Birck, M.R., Rinaldo-Matthis, A., et al., Taylor, E.A., Almo, S.C., and Schramm, V.L., in 2007 in a paper entitled "Neighboring group participation in the transition state of human purine nucleoside phosphorylase." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.28 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Purine nucleoside phosphorylase. This molecule has the UniProt identifier P00491 (PNPH_HUMAN)search. The sample contained 289 residues which is 100% of the natural sequence. Out of 289 residues 282 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms homotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Purine nucleoside phosphorylase P00491 (1-289) (PNPH_HUMAN)search Homo sapienssearch 100% 289 97%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P00491 (1 - 289) Purine nucleoside phosphorylase Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P00491) Rossmann foldsearch PF01048: Phosphorylase superfamilysearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P00491) purine-nucleoside phosphorylase activitysearch drug bindingsearch phosphate ion bindingsearch transferase activitysearch purine nucleobase bindingsearch catalytic activitysearch nucleoside bindingsearch transferase activity, transferring glycosyl groupssearch transferase activity, transferring pentosyl groupssearch nucleobase-containing compound metabolic processsearch nucleoside metabolic processsearch urate biosynthetic processsearch immune responsesearch interleukin-2 secretionsearch positive regulation of alpha-beta T cell differentiationsearch response to drugsearch NAD biosynthesis via nicotinamide riboside salvage pathwaysearch purine nucleobase metabolic processsearch nicotinamide riboside catabolic processsearch nucleobase-containing small molecule metabolic processsearch positive regulation of T cell proliferationsearch small molecule metabolic processsearch inosine catabolic processsearch purine nucleotide catabolic processsearch purine-containing compound salvagesearch cytosolsearch cytoplasmsearch extracellular exosomesearch cytoskeletonsearch intracellularsearch

Chain InterPro annotation
A Nucleoside phosphorylase domainsearch PNP/MTAP phosphorylasesearch Purine nucleoside phosphorylasesearch Purine nucleoside phosphorylase I, inosine/guanosine-specificsearch Purine phosphorylase, family 2, conserved sitesearch