› 2zyc

X-ray diffraction
1.74Å resolution

Crystal structure of peptidoglycan hydrolase from Sphingomonas sp. A1

Released:
DOI: 10.2210/pdb2zyc/pdb
PDB entry 2zyc coloured by chain, front view.
PDB entry 2zyc coloured by chain, side view.
PDB entry 2zyc coloured by chain, top view.
Source organism: Sphingomonas sp. A1
Primary publication:
Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ.
Hashimoto W, Ochiai A, Momma K, Itoh T, Mikami B, Maruyama Y, Murata K
Biochem Biophys Res Commun 381 16-21 (2009)
PMID: 19351587

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan hydrolase FlgJ Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 18.73 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7XH69 (Residues: 152-313; Coverage: 52%)
Gene name: flgJ
Sequence domains: Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P43212
Unit cell:
a: 54.28Å b: 54.28Å c: 102.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
1 more

6 mentions without citation

Cell Wall Hydrolases in Bacteria: Insight on the Diversity of Cell Wall Amidases, Glycosidases and Peptidases Toward Peptidoglycan.
Vermassen et al. (2019)
5 more