› 2zw0

X-ray diffraction
1.4Å resolution

Crystal structure of a Streptococcal protein G B1 mutant

Released:
DOI: 10.2210/pdb2zw0/pdb
PDB entry 2zw0 coloured by chain, front view.
PDB entry 2zw0 coloured by chain, side view.
PDB entry 2zw0 coloured by chain, top view.
Source organism: Finegoldia magna
Primary publication:
Optimizing pH response of affinity between protein G and IgG Fc: how electrostatic modulations affect protein-protein interactions.
Watanabe H, Matsumaru H, Ooishi A, Feng Y, Odahara T, Suto K, Honda S
J Biol Chem 284 12373-83 (2009)
PMID: 19269963

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IgG-binding B domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 57 amino acids
Theoretical weight: 6.4 KDa
Source organism: Finegoldia magna
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53291 (Residues: 328-384; Coverage: 13%)
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2221
Unit cell:
a: 25.764Å b: 36.36Å c: 93.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.224
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cancer acidity: An ultimate frontier of tumor immune escape and a novel target of immunomodulation.
Huber et al. (2017)
3 more

1 mention without citation

Optimizing pH response of affinity between protein G and IgG Fc: how electrostatic modulations affect protein-protein interactions.
Watanabe et al. (2009)